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| Status |
Public on Apr 27, 2017 |
| Title |
Piwi is required during Drosophila embryogenesis to license dual-strand piRNA clusters for transposon repression in adult ovaries [ChIP-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Most piRNAs in the Drosophila female germline are transcribed from heterochromatic regions called dual-strand piRNA clusters. Histone 3 lysine 9 trimethylation (H3K9me3) is required for licensing piRNA production by these clusters. However, it is unclear when and how they acquire this permissive heterochromatic state. Although it has been suggested that piRNA cluster licensing is Piwi-independent, here we show that transient Piwi depletion in Drosophila embryos, using a refined knock-down system, results in H3K9me3 decrease at piRNA clusters. This is accompanied by aberrant maturation of piRNA precursor transcripts, accumulation of transposable element transcripts and female sterility. Conversely, Piwi knock-down at later developmental stages does not disturb piRNA cluster licensing, as previously reported. These results indicate that the identity of piRNA clusters is epigenetically acquired in a Piwi-dependent manner during a limited embryonic development window, which is reminiscent of the widespread genome reprogramming occurring during early mammalian zygotic development.
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| Overall design |
3 independate biological triplicates of Chip H3K9me3 input and IP were performed for each of the 2 genotypes (piwi-embKD and white_embKD).
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| Contributor(s) |
Akkouche A, Mugat B, Barckmann B, Varela-Chavez C, Li B, Raffel R, Pélisson A, Chambeyron S |
| Citation(s) |
28457744 |
| |
| Submission date |
Jun 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
SEVERINE CHAMBEYRON |
| E-mail(s) |
severine.chambeyron@igh.cnrs.fr
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| Organization name |
CNRS
|
| Department |
Institute of Human Genetics
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| Lab |
Non-coding RNA, epigenetics and genome stability
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| Street address |
141, rue de la Cardonille
|
| City |
MONTPELLIER |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE83238 |
Piwi is required during Drosophila embryogenesis to license dual-strand piRNA clusters for transposon repression in adult ovaries |
|
| Relations |
| BioProject |
PRJNA325319 |
| SRA |
SRP076399 |
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