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| Status |
Public on Aug 14, 2017 |
| Title |
NFIA Controls the Brown Fat Gene Program by Co-Localizing with PPARgamma at Cell-Type-Specific Enhancers (chromatin) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a novel transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the adipogenic master regulator, PPARgamma, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARgamma, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Finally, expression of NFIA and the brown-fat-specific genes is positively correlated in human brown fat. These results indicate that NFIA is a key transcriptional regulator of brown fat and exerts its effects by co-localizing with PPARg at cell-type-specific enhancers.
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| Overall design |
FAIRE-seq analysis of murine BAT, iWAT and eWAT, ChIP-seq analysis in immortalized brown adipocytes and NFIA-expressing C2C12 myoblasts, and ATAC-seq analysis in immortalized brown adipocytes.
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| Contributor(s) |
Hiraike Y, Waki H, Nakamura M, Suzuki K, Nakaki R, Yamamoto S, Tsutsumi S, Aburatani H, Yamauchi T, Kadowaki T |
| Citation(s) |
28812581 |
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| Submission date |
Jun 27, 2016 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hironori Waki |
| E-mail(s) |
hwaki-tky@umin.ac.jp
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| Organization name |
The University of Tokyo
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| Street address |
Hongo 7-3-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
1138655 |
| Country |
Japan |
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| Platforms (3) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (24)
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| GSM2218582 |
NFI_ChIP-seq_brown_adipocytes_day0 |
| GSM2218583 |
NFI_ChIP-seq_brown_adipocytes_day6 |
| GSM2218584 |
PPARg_ChIP-seq_brown_adipocytes_day0_rep1 |
| GSM2218585 |
PPARg_ChIP-seq_brown_adipocytes_day0_rep2 |
| GSM2218586 |
PPARg_ChIP-seq_brown_adipocytes_day6_rep1 |
| GSM2218587 |
PPARg_ChIP-seq_brown_adipocytes_day6_rep2 |
| GSM2218588 |
ATAC-seq_brown_adipocytes_day6_rep1 |
| GSM2218589 |
ATAC-seq_brown_adipocytes_day6_rep2 |
| GSM2218590 |
NFIA_ChIP-seq_NFIA-expressing_C2C12_myoblasts_day0 |
| GSM2218591 |
NFIA_ChIP-seq_NFIA-expressing_C2C12_myoblasts_day7 |
| GSM2546549 |
ATAC-seq_brown_adipocytes_day0_rep1 |
| GSM2546550 |
H3K27Ac_ChIP-seq_brown_adipocytes_day0 |
| GSM2546551 |
CEBPalpha_ChIP-seq_brown_adipocytes_day0 |
| GSM2546552 |
CEBPbeta_ChIP-seq_brown_adipocytes_day0 |
| GSM2546553 |
H3K27Ac_ChIP-seq_brown_adipocytes_day6 |
| GSM2546554 |
CEBPalpha_ChIP-seq_brown_adipocytes_day6 |
| GSM2546555 |
CEBPbeta_ChIP-seq_brown_adipocytes_day6 |
| GSM2546556 |
NFI_ChIP-seq_white_adipocytes_day7 |
| GSM2546557 |
PPARg_ChIP-seq_white_adipocytes_day7 |
| GSM2735134 |
EBF2_ChIP-seq_brown_adipocytes_day0 |
| GSM2735135 |
EBF2_ChIP-seq_brown_adipocytes_day6 |
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| This SubSeries is part of SuperSeries: |
| GSE83764 |
NFIA Controls the Brown Fat Gene Program by Co-Localizing with PPARgamma at Cell-Type-Specific Enhancers |
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| Relations |
| BioProject |
PRJNA326936 |
| SRA |
SRP077281 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83757_ATAC-seq_brown_adipocytes_day6_rep1_2.bigWig |
256.2 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_FAIRE_BAT.bdg.bedgraph.gz |
359.6 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE83757_FAIRE_eWAT.bdg.bedgraph.gz |
180.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE83757_FAIRE_iWAT.bdg.bedgraph.gz |
203.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE83757_NFIA_ChIP-seq_NFIA-expressing_C2C12_myoblasts_day0.bigWig |
608.6 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_NFIA_ChIP-seq_NFIA-expressing_C2C12_myoblasts_day7.bigWig |
626.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_NFI_ChIP-seq_brown_adipocytes_day0.bigWig |
273.1 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_NFI_ChIP-seq_brown_adipocytes_day6.bigWig |
198.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_PPARg_ChIP-seq_brown_adipocytes_day0_rep1_2.bigWig |
173.7 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_PPARg_ChIP-seq_brown_adipocytes_day6_rep1_2.bigWig |
502.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE83757_RAW.tar |
3.3 Gb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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