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| Status |
Public on May 28, 2017 |
| Title |
miR-34a and miR-34b/c suppress intestinal tumorigenesis in ApcMin/+ Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
miR-34a and miR-34b/c genes are frequently epigenetically silenced in primary CRCs. However, the in vivo relevance of miR-34a/b/c for suppression of intestinal tumor formation has not been analyzed by genetic approaches. ApcMin/+ mice with deletion of the miR-34a and miR-34b/c genes were generated and analyzed. The mRNA expression profiles of intestinal adenomas with and without functional miR-34a/b/c genes were compared.
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| Overall design |
miR-34a/b/c deficient ApcMin/+ mice and wild-type ApcMin/+ mice were sacrificed at 18 weeks of age. 3 tumor RNA samples were obtained for each genotype; each tumor RNA sample represented a pool of 3 tumors isolated from the same mouse.
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| Contributor(s) |
Jiang L, Hermeking H |
| Citation(s) |
28363996 |
| |
| Submission date |
Jul 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Longchang Jiang |
| E-mail(s) |
Longchang.Jiang@med.uni-muenchen.de
|
| Phone |
+49 89 2180 73724
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| Organization name |
Ludwig-Maximilians-University München
|
| Department |
Institute of Pathology
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| Lab |
Experimental and Molecular Pathology
|
| Street address |
Thalkirchner Strasse 36
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| City |
Munich |
| ZIP/Postal code |
80337 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA328138 |
| SRA |
SRP078057 |
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