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GEO help: Mouse over screen elements for information. |
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Status |
Public on Oct 20, 2016 |
Title |
Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Dendritic cell subsets purified from skin-draining lymph nodes of C57BL/6J mice following intradermal injection of non-viable Nippostrongylus brasiliensis (Nb) or topical application of Dibutyl Phthalate-FITC. Results provide insight into the genetic programme induced in DC exposed to Th2 stimuli.
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Overall design |
CD11b+, TN and CD103+ (Nb only) migratory DC subsets were flow sorted from skin-draining lymph nodes following treatment with Th2 stimuli or respective controls. Three independent replicates were generated for each DC population from pooled lymph nodes in three independent flow cytometry sorting experiments.
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Contributor(s) |
Ronchese F, McConnell M, Connor L, Tang S, Ochiai S, Lamiable O, Eccles D, Smith AA |
Citation(s) |
27913566 |
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Submission date |
Oct 20, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Franca Ronchese |
E-mail(s) |
fronchese@malaghan.org.nz
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Phone |
+64 4 499 6914
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Organization name |
Malaghan Instititute of Medical Research
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Department |
Immune Cell Biology
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Street address |
Kelburn Parade
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City |
Wellington |
ZIP/Postal code |
6012 |
Country |
New Zealand |
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Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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Samples (30)
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GSM2356908 |
PBS, TN, Replicate 1 |
GSM2356909 |
PBS, TN, Replicate 2 |
GSM2356910 |
PBS, TN, Replicate 3 |
GSM2356911 |
PBS, CD103, Replicate 1 |
GSM2356912 |
PBS, CD103, Replicate 2 |
GSM2356913 |
PBS, CD103, Replicate 3 |
GSM2356914 |
Nb, CD11b, Replicate 1 |
GSM2356915 |
Nb, CD11b, Replicate 2 |
GSM2356916 |
Nb, CD11b, Replicate 3 |
GSM2356917 |
Nb, TN, Replicate 1 |
GSM2356918 |
Nb, TN, Replicate 2 |
GSM2356919 |
Nb, TN, Replicate 3 |
GSM2356920 |
Nb, CD103, Replicate 1 |
GSM2356921 |
Nb, CD103, Replicate 2 |
GSM2356922 |
Nb, CD103, Replicate 3 |
GSM2356923 |
Untreated, CD11b, Replicate 1 |
GSM2356924 |
Untreated, CD11b, Replicate 2 |
GSM2356925 |
Untreated, CD11b, Replicate 3 |
GSM2356926 |
Untreated, TN, Replicate 1 |
GSM2356927 |
Untreated, TN, Replicate 2 |
GSM2356928 |
Untreated, TN, Replicate 3 |
GSM2356929 |
DBP-FITC, CD11b, Replicate 1 |
GSM2356930 |
DBP-FITC, CD11b, Replicate 2 |
GSM2356931 |
DBP-FITC, CD11b, Replicate 3 |
GSM2356932 |
DBP-FITC, TN, Replicate 1 |
GSM2356933 |
DBP-FITC, TN, Replicate 2 |
GSM2356934 |
DBP-FITC, TN, Replicate 3 |
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Relations |
BioProject |
PRJNA349476 |
SRA |
SRP091875 |
Supplementary file |
Size |
Download |
File type/resource |
GSE88998_JEMsampleFiltered_collapsed_counts_2016-May-05.csv.gz |
2.0 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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