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Series GSE88998 Query DataSets for GSE88998
Status Public on Oct 20, 2016
Title Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Dendritic cell subsets purified from skin-draining lymph nodes of C57BL/6J mice following intradermal injection of non-viable Nippostrongylus brasiliensis (Nb) or topical application of Dibutyl Phthalate-FITC. Results provide insight into the genetic programme induced in DC exposed to Th2 stimuli.
 
Overall design CD11b+, TN and CD103+ (Nb only) migratory DC subsets were flow sorted from skin-draining lymph nodes following treatment with Th2 stimuli or respective controls. Three independent replicates were generated for each DC population from pooled lymph nodes in three independent flow cytometry sorting experiments.
 
Contributor(s) Ronchese F, McConnell M, Connor L, Tang S, Ochiai S, Lamiable O, Eccles D, Smith AA
Citation(s) 27913566
Submission date Oct 20, 2016
Last update date May 15, 2019
Contact name Franca Ronchese
E-mail(s) fronchese@malaghan.org.nz
Phone +64 4 499 6914
Organization name Malaghan Instititute of Medical Research
Department Immune Cell Biology
Street address Kelburn Parade
City Wellington
ZIP/Postal code 6012
Country New Zealand
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (30)
GSM2356905 PBS, CD11b, Replicate 1
GSM2356906 PBS, CD11b, Replicate 2
GSM2356907 PBS, CD11b, Replicate 3
Relations
BioProject PRJNA349476
SRA SRP091875

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88998_JEMsampleFiltered_collapsed_counts_2016-May-05.csv.gz 2.0 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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