|
| Status |
Public on Jun 03, 2017 |
| Title |
RNA-seq analysis of ESR1 mutations in T47D and MCF7 cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Transcriptome analysis of ESR1 mutant cells was performed via sequencing total RNA in T47D and MCF7 cell lines containing Y537S and D538G mutations.
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| |
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| Overall design |
Methods: Individual ESR1 WT and mutant T47D and MCF7 clones were hormone deprived in CSS for three days, pooled, and plated in quadruplicates in 6-well plates. The cells were treated with veh or 1nM E2 for 24 hrs, RNA was isolated and subjected to sequencing. Differential expression analysis was performed by DEseq2 and R was used to conduct statistical analysis tests
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| |
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| Contributor(s) |
Bahreini A, Oesterreich S |
| Citation(s) |
28535794 |
| |
| Submission date |
Nov 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Amir Bahreini |
| E-mail(s) |
bahreinia@upmc.edu
|
| Phone |
5712444045
|
| Organization name |
University of Pittsburgh
|
| Department |
Human Genetics
|
| Street address |
204 Craft Ave A430
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (48)
|
|
| Relations |
| BioProject |
PRJNA353646 |
| SRA |
SRP093386 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89888_resD538G_E2vVeh_MCF7.csv.gz |
778.5 Kb |
(ftp)(http) |
CSV |
| GSE89888_resD538G_E2vVeh_T47D.csv.gz |
789.2 Kb |
(ftp)(http) |
CSV |
| GSE89888_resVeh_WTvD538G_MCF7.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE89888_resVeh_WTvD538G_T47D.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE89888_resVeh_WTvY537S_MCF7.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE89888_resVeh_WTvY537S_T47D.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE89888_resWT_E2vVeh_MCF7.csv.gz |
795.3 Kb |
(ftp)(http) |
CSV |
| GSE89888_resWT_E2vVeh_T47D.csv.gz |
789.4 Kb |
(ftp)(http) |
CSV |
| GSE89888_resY537S_E2vVeh_MCF7.csv.gz |
780.3 Kb |
(ftp)(http) |
CSV |
| GSE89888_resY537S_E2vVeh_T47D.csv.gz |
786.1 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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