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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 15, 2008 |
| Title |
Transcriptional analysis of BCR-triggered immature and mature mouse B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The B cell antigen receptor (BCR) is a signaling complex that mediates differentiation stage-specific cell fate decisions in B lymphocytes. While several studies have evidenced differences in signal transduction components as being key to contrasting phenotypic outcomes, little is known about the differential BCR-triggered gene transcription. Here we defined transcriptional changes underlying BCR-induced apoptosis and proliferation of immature and mature B cells, respectively. These findings provide the first insights into the early transcriptional events leading to deletion or clonal expansion of B cells upon antigen recognition.
Keywords: cell type comparison, development or differentiation design, time course, compound treatment design, microarray experiment record
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| Overall design |
Total RNA was extracted from immature (IM) and mature (M) B cells both freshly isolated (0h) and stimulated with (BCR) or without (ctrl) anti-μ F(ab’)2 for 2, 8 or 24h. Following RNA extraction, one round of mRNA amplification was applied using the MessageAmpTM Kit (Ambion). All samples, including the amplified Universal Mouse Reference RNA (Stratagene) which served as a reference at each hybridization, were reverse-transcribed and indirectly labeled with Cy3 and Cy5 (Amersham). Quality was controlled by performing 6 or 8 hybridizations of each sample to the cDNA arrays using a dye-swap strategy.
94 arrays were used for hybridization in total.
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| Contributor(s) |
Murn J, Gidrol X |
| Citation(s) |
19075289, 19607732 |
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| Submission date |
Oct 03, 2007 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Olivier ALIBERT |
| E-mail(s) |
olivier.alibert@cea.fr
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| Organization name |
CEA
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| Department |
IRCM
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| Lab |
LEFG
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| Street address |
2 rue Gaston Crémieux
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| City |
Evry |
| ZIP/Postal code |
91057 |
| Country |
France |
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| Platforms (2) |
| GPL5950 |
CEA - DSV/IRCM/LEFG : NIA Version 1 |
| GPL5951 |
CEA - DSV/IRCM/LEFG : NIA Version 2 |
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| Samples (94)
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| Relations |
| BioProject |
PRJNA102805 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9215_RAW.tar |
104.8 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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