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| Status |
Public on Feb 03, 2017 |
| Title |
Expression Analysis of 2D, 3D Quiescent, and 3D Activated Pancreatic Stellate Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study used Illumina single-end RNA-sequencing to examine gene expression differences between 2 mouse-derived pancreatic stellate cell lines (PSC4, PSC5) grown either in 2D monolayers, as 3D quiescent cultures, or as 3D activated transwell cocultures with 2 mouse tumor-derived pancreatic ductal organoid lines (T4, T5). Mouse pancreatic stellate cell (PSC) lines were derived from the pancreata of wild-type C57Bl/6J mice. Mouse tumor organoid lines were derived from mouse pancreata containing pancreatic ductal adenocarcinoma (PDAC) from the KrasLSL-G12D; Trp53LSL-R162H; Pdx1-Cre mouse model. We measured genes differentially expressed among 2D, quiescent, and 3D activated PSCs that may reflect the expression changes of heterogeneous CAF population in pancreatic tumors.
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| Overall design |
Gene expression comparison of 2 murine pancreatic stellate cell lines grown either in 2D monolayers, 3D quiescent cultures, or 3D activated transwell cocultures with 2 murine pancreatic ductal adenocarcinoma-derived pancreatic ductal organoid lines.
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| Contributor(s) |
Tuveson DA, Baker LA, Handly-Santana A |
| Citation(s) |
28232471 |
| |
| Submission date |
Jan 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
David A. Tuveson |
| Organization name |
Cold Spring Harbor Laboratory
|
| Department |
Cancer Center
|
| Lab |
The Tuveson Laboratory
|
| Street address |
1 Bungtown Rd.
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA360599 |
| SRA |
SRP096327 |
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