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| Status |
Public on Jan 20, 2017 |
| Title |
Salmonella Typhimurium fimZ mutant |
| Platform organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
| Sample organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Expression profiling by array
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| Summary |
Salmonella enterica serovar Typhimurium produces type 1 fimbriae on the outer membrane and such hair-like appendages is proposed to play a significant role in the attachment of the bacteria to host cells and tissues. S. Typhimurium cultured in static broth favors expression of type 1 fimbriae. Conversely, solid agar medium represses type 1 fimbrial expression. Production of type 1 fimbriae is cooperatively regulated by fimZ, fimY, stm0551, fimW, and fimU within the fim gene cluster. FimZ belongs to the response regulator of the two-component regulatory system in bacteria and functions as a DNA binding protein. FimZ can bind to the promoter of the fimbrial major subunit gene fimA to activate its expression and produce type 1 fimbriae. A fimZ mutant in LB5010 strain constructed by allelic exchange was found to be non-fimbriate. Total RNA was extracted from the parental LB5010 and the fimZ mutant strain cultured in static broth and analyzed by hybridization to a S. Typhimurium LT2 DNA microarray. Transcriptomic analysis indicated that the stress response related gene cpxP, soxS and type 1 fimbriae related genes were down-regulated, whereas plasmid-encoded fimbriae, flagella associated genes and virulence genes like virK and mgtC were up-regulated in the fimZ mutant strain. It is possible that FimZ protein may play a role to interact with other genes besides fim genes. Mechanisms of this crosstalk warrant further elucidation.
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| Overall design |
RNA transcript of Salmonella Typhimurium LB5010 strain comparing wild-type with fimZ mutant. Two-condition experiment, wild-type vs. fimZ mutant strain.
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| Contributor(s) |
Yeh K, Chang C |
| Citation missing |
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| Submission date |
Jan 19, 2017 |
| Last update date |
Jan 21, 2017 |
| Contact name |
Chang Chieh-Hsiang |
| E-mail(s) |
r04629010@ntu.edu.tw
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| Organization name |
National Taiwan University
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| Street address |
No. 1, Sec. 4, Roosevelt Rd
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| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL22962 |
Agilent-015906 Salmonella enterica 4x44K microarray (Feature Number version) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA362647 |
