|
| Status |
Public on Nov 06, 2007 |
| Title |
Changes in gene expression in response to heme deficiency and hypoxia |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
We addressed changes in gene expression profile in response to
reduced oxygen tension in wild type or heme depletion caused by
deletion of heme biosynthesis gene hem1
Keywords: Response to heme depletion and hypoxia
|
| |
|
| Overall design |
I.Heme depletion.The hem1 delta strain was grown for 6 hrs in YPD media supplemented with 250 uM ALA. Cells were washed three times with water to remove excess ALA. Cells were then inoculated in duplicates into 15 ml cultures of
YPD media that was supplemented either with or without ALA. Cultures were induced for 16 h before 5 ml of cells were harvested and washed
three times in DEPC water. The culture density at the time of harvest
was approximately OD600=0.5.
II.Hypoxia. BY45742 strain was grown for 6 hrs in
YPD medium and then inoculated in 15 ml the same medium and grown
under normoxic or hypoxic conditions for 12 hrs. To create hypoxic
conditions, cultures were incubated in an anaerobic chamber using a
BBL-GasPak (BD Biosciences).
|
| |
|
| Contributor(s) |
Protchenko O, Philpott C |
| Citation(s) |
18326586 |
| |
| Submission date |
Nov 05, 2007 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Olga Protchenko |
| E-mail(s) |
olgap@intra.niddk.nih.gov
|
| Organization name |
National Institutes of health
|
| Department |
NIDDK
|
| Street address |
10 Center Dr, Bldg.10-Rm.9B-05
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
|
| Samples (8)
|
| GSM241146 |
hem1 strain grown in YPD with 250 uM ALA (08-15-06_Philpott_YG_S98_1) |
| GSM241147 |
WT strain grown in YPD under Hypoxia (08-15-06_Philpott_YG_S98_10) |
| GSM241148 |
WT strain grown in YPD under Hypoxia (08-15-06_Philpott_YG_S98_11) |
| GSM241149 |
hem1 strain grown in YPD with 250 uM ALA (08-15-06_Philpott_YG_S98_2) |
| GSM241150 |
hem1 strain grown in YPD w/o ALA (08-15-06_Philpott_YG_S98_3) |
| GSM241151 |
hem1 strain grown in YPD w/o ALA (08-15-06_Philpott_YG_S98_4) |
| GSM241152 |
WT strain grown in YPD under Normoxia (08-15-06_Philpott_YG_S98_8) |
| GSM241153 |
WT strain grown in YPD under Normoxia (08-15-06_Philpott_YG_S98_9) |
|
| Relations |
| BioProject |
PRJNA103313 |
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