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| Status |
Public on Jan 24, 2018 |
| Title |
Hemicastration induced spermatogenesis related DNA methylation and gene expression changes in mice testis |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Hemicastration is unilateral orchiectomy to remove an injured testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we monitored hemicastrated mice and found no significant difference of growth or testosterone (P > 0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis were investigated by RRBS. Substantial genes harbored differentially methylated regions in gene bodies, and were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 DEGs involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that PSMC3IP, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Our results demonstrated that hemicastration-induced compensatory response maintained normal growth and fertility in mice.
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| Overall design |
A total of six right testis of healthy male KM mice with body maturation were used in this study: three individuals were performed prepubertal hemicastration to remove left testis; At the same time, three counterparts were performed shame surgery.
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| Contributor(s) |
Wang Y |
| Citation(s) |
29059727 |
| |
| Submission date |
Mar 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yixin Wang |
| E-mail(s) |
yixin25@ualberta.ca
|
| Phone |
8615378680683
|
| Organization name |
Sichuan Agricultural University
|
| Department |
Institute of Animal Genetics and Breeding, College of Animal Science and Technology
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| Lab |
Lab of animal genomics
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| Street address |
211 huimin street
|
| City |
chengdu |
| State/province |
sichuan |
| ZIP/Postal code |
611130 |
| Country |
China |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE95694 |
Hemicastration induced spermatogenesis related DNA methylation and gene expression changes in mice testis |
|
| Relations |
| BioProject |
PRJNA377981 |
| SRA |
SRP101398 |
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