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| Status |
Public on Apr 07, 2017 |
| Title |
Genome-wide analysis of the differentially expressed genes between a butanol-producing wild type strain and a pykA mutant during fermentation |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To analyze the change of transcription pattern and differentially expressed genes caused by pykA inactivation as the pykA mutant showed a longer growth cycle, increased butanol titer and less pyruvate accumulation. Some new target genes may be used for further metabolic engineering.
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| Overall design |
The total RNA of strain EB216 and EB216-∆pykA (EB222) was isolated in different time points in the fermentation process at 6 h, 12 h, 24 h, and 48 h. Samples of strain EB216 were used as control samples for strain EB216-∆pykA at the respective time. Illumina Hiseq2000 platform was employed for RNA-Seq.
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| Contributor(s) |
Zhao C, Lin Z, Dong H, Zhang Y, Li Y |
| Citation(s) |
28363967 |
| |
| Submission date |
Mar 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chunhua Zhao |
| E-mail(s) |
jeil0901@163.com
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| Organization name |
Institute of Microbiology, Chinese Academy of Sciences
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| Street address |
No.1 Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL14548 |
Illumina HiSeq 2000 (Escherichia coli) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA378992 |
| SRA |
SRP101799 |
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