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| Status |
Public on Sep 07, 2017 |
| Title |
ENAP1 is involved in ethylene response |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report that ethylene regulate the ENAP1 binding. We perform a dual cross-linking procedure using the additional cross-linker ethylene glycol bis-succinimidylsuccinate (EGS; Thermo Scientific) along with formaldehyde according to (Pedmale et al. 2016) for Chip-sequencing (ChIPseq) of ENAP1, as well as ENAP1 ChIP- EIN2 reChIPseq (ChIP-reChIPseq), which were used chromatins isolated from 3-day old etiolated Col-0 seedlings treated with ethylene or air gas. Results show that ENAP1 is involved in the EIN2 dependent ethylene response.
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| |
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| Overall design |
Examination of 2 different protein binding in 1 Arabidopsis lines which under 2 different treatment.
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| Contributor(s) |
Qiao H, Wang L, Zhang F |
| Citation(s) |
28874528 |
| Submission date |
Mar 31, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hong Qiao |
| Organization name |
The University of Texas at Austin
|
| Department |
Department of Molecular Biosciences
|
| Lab |
Qiao lab
|
| Street address |
2506 speedway, NMS5.324
|
| City |
Austin |
| State/province |
TEXAS |
| ZIP/Postal code |
78731 |
| Country |
USA |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA381206 |
| SRA |
SRP102817 |
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