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| Status |
Public on Jul 28, 2017 |
| Title |
Lethality of MalE-LacZ hybrid protein shares mechanistic attributes with oxidative component of antibiotic lethality |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli str. K-12 substr. MC4100 |
| Experiment type |
Expression profiling by array
|
| Summary |
This study examines stress responses following lethal induction of a MalE-LacZ hybrid protein
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| |
|
| Overall design |
E coli MC4100 cells bearing a MalE-LacZ hybrid protein under a maltose promoter (MM18) were grown in M63 medium and induced with 0.2% maltose. Cells were incubated for at 37C with shaking and sampled hourly over 5 hr for RNA extraction.
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| |
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| Contributor(s) |
Takahashi N, Gruber CC, Yang JH, Collins JJ, Walker GC |
| Citation(s) |
28794281 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA021615 |
Mutagenesis and Repair of DNA |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
GRAHAM C WALKER |
| K99 GM118907 |
Effects of Host Metabolic Variation on Antibiotic Susceptibility |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
Jason Hung-Ying Yang |
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| |
| Submission date |
May 03, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jason H Yang |
| E-mail(s) |
jasonhy@mit.edu
|
| Organization name |
MIT / Broad Institute
|
| Department |
Biological Engineering
|
| Lab |
James Collins
|
| Street address |
415 Main St, Rm 2017
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
|
| Samples (36)
|
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| Relations |
| BioProject |
PRJNA385309 |
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