|
| Status |
Public on Dec 01, 2012 |
| Title |
Cardiac progenitor c-kit cells_Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Flow cytomtery based sorting of ckit+ cells from heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Cardiac progenitor c-kit cells
genotype: Normal
|
| Growth protocol |
Matrigel coated culture dishes and mTeSR
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen microkit; small RNAs preserved by using buffer from miRNAeasy kit; cDNA was synthesised and amplified using a WT-Ovation™ Pico RNA Amplification System
|
| Label |
Cy5
|
| Label protocol |
Amplified cDNA was labeled with Cy5™ NHS ester (GEH Lifesciences)
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized at 42°C overnight using an SC chamber on the MAUI® Hybridization System (BioMicro®) with 1X OneArray® Hybridization Buffer (Phalanx Biotech), 0.01 mg/ml sheared salmon sperm DNA (Promega), at a concentration of 0.025 mg/ml labeled target. After hybridization, the arrays were washed according to the OneArray® protocol. cDNA microarray analysis was performed using the OneArray® Mouse Whole Genome Array (Phalanx Biotech, Belmont, CA).
|
| Scan protocol |
Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format. Although the chip is two color, we only used Cy5 as the data channel.
|
| Description |
Gene expression data from CPCs
|
| Data processing |
The raw intensities were normalized with center median scaling by Phanlax
|
| |
|
| Submission date |
Sep 26, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Leng Han |
| E-mail(s) |
hanleng0926@hotmail.com
|
| Organization name |
Stanford University
|
| Street address |
265 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL13692 |
| Series (1) |
| GSE41175 |
Dissecting the Molecular Relationship Between Cardiac And Bone Marrow-Derived Progenitor Cells |
|
