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Sample GSM101683 Query DataSets for GSM101683
Status Public on May 10, 2006
Title U387_1
Sample type RNA
 
Source name Tumor cell line U387
Organism Homo sapiens
Characteristics commonly used glioma line
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description Tumor cell line U387, cultured in serum
Data processing Affymetrix GCOS 1.2 with default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Mar 24, 2006
Last update date Aug 28, 2018
Contact name Howard Fine
E-mail(s) hfine@mail.nih.gov
Phone 301-402-6383
Fax 301-480-2246
Organization name National Cancer Institute
Department Neurological Disorders and Stroke
Street address 9030 Old Georgetown Rd
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL570
Series (1)
GSE4536 Tumor stem cells more closely mirror the phenotype and genotype of primary human tumors than do cancer cell lines
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 2709.5 P 0.000266
1053_at 2625.6 P 0.000266
117_at 226.4 P 0.043968
121_at 1014.1 P 0.004863
1255_g_at 54.2 A 0.397994
1294_at 333.4 P 0.048995
1316_at 152.5 P 0.003067
1320_at 144.9 A 0.250724
1405_i_at 3.6 A 0.995409
1431_at 48.0 A 0.089405
1438_at 633.2 P 0.005643
1487_at 652.1 P 0.031336
1494_f_at 205.4 A 0.098054
1598_g_at 1696.3 P 0.003585
160020_at 798.6 P 0.017001
1729_at 1020.0 P 0.001602
1773_at 416.1 P 0.039365
177_at 236.0 P 0.006532
179_at 1108.9 P 0.039365
1861_at 638.0 P 0.000959

Total number of rows: 54613

Table truncated, full table size 1465 Kbytes.




Supplementary file Size Download File type/resource
GSM101683.CEL.gz 5.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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