|
| Status |
Public on Dec 31, 2013 |
| Title |
Cd Replicate3 96 Hour Female |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Female zebrafish liver tissue
|
| Organism |
Danio rerio |
| Characteristics |
treatment: cadmium
tissue: liver
time: 96 h
gender: female
strain: Singapore wild-type
|
| Treatment protocol |
Female zebrafish were exposed to 30 µg/L Cadmium for 8, 24, 48, and 96 hours at room temperature.
|
| Growth protocol |
Zebrafish were maintained at room temperature (26°C + 1°C)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of tissue samples were extracted using Trizol reagent (Invitrogen) according to the manufacturers instructions.
|
| Label |
Cy5
|
| Label protocol |
For fluorescence labeling of cDNAs, 10 µg of total RNA from the reference and experimental samples were reverse transcribed in the presence of Cy3-dUTP and Cy5-dUTP (Amersham Inc.), respectively. Reference RNA was obtained by pooling equal amount of male and female total RNA extracted from liver tissues of wild-type zebrafish. Labeled cDNA were concentrated, and resuspended in DIG EasyHyb (Roche Applied Science) buffer for hybridization.
|
| |
|
| Channel 2 |
| Source name |
Reference RNA was obtained by pooling equal amount of male and female total RNA extracted from liver tissues of wild-type zebrafish.
|
| Organism |
Danio rerio |
| Characteristics |
sample type: Reference RNA was obtained by pooling equal amount of male and female total RNA extracted from liver tissues of wild-type zebrafish.
gender: male and female
tissue: liver
strain: Singapore wild-type
|
| Treatment protocol |
Female zebrafish were exposed to 30 µg/L Cadmium for 8, 24, 48, and 96 hours at room temperature.
|
| Growth protocol |
Zebrafish were maintained at room temperature (26°C + 1°C)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of tissue samples were extracted using Trizol reagent (Invitrogen) according to the manufacturers instructions.
|
| Label |
Cy3
|
| Label protocol |
For fluorescence labeling of cDNAs, 10 µg of total RNA from the reference and experimental samples were reverse transcribed in the presence of Cy3-dUTP and Cy5-dUTP (Amersham Inc.), respectively. Reference RNA was obtained by pooling equal amount of male and female total RNA extracted from liver tissues of wild-type zebrafish. Labeled cDNA were concentrated, and resuspended in DIG EasyHyb (Roche Applied Science) buffer for hybridization.
|
| |
|
| |
| Hybridization protocol |
Reference RNA (labelled with Cy3) is co-hybridized with RNA samples extracted either from treated or control fish (labelled with Cy5) on a glass array spotted with Zf_16.5K_oligo_COMP_v3Apx zebrafish oligo probes.
|
| Scan protocol |
The arrays were scanned using the GenePix 4000B microarray scanner (Axon Instruments, USA) and the generated images with their fluorescence signal intensities were analyzed using GenePix Pro 4.0 image analysis software (Axon Instruments, USA).
|
| Description |
Biological replicate 2 of 3: Cadmium-treated at 96 Hr
|
| Data processing |
The data were global median normalized. Normalized ratio of means defined by CH1/CH2
|
| |
|
| Submission date |
Oct 16, 2012 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Choong Yong UNG |
| E-mail(s) |
matucy@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Department of Biological Sciences
|
| Street address |
14 Science Drive 4
|
| City |
Singapore |
| ZIP/Postal code |
117543 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL2715 |
| Series (2) |
| GSE41622 |
Toxicogenomics and phenotypic characterization of adult female zebrafish liver treated with cadmium (II) |
| GSE41625 |
Toxicogenomics and phenotypic characterization of adult female and zebrafish liver treated with cadmium (II) |
|
