|
| Status |
Public on Dec 31, 2013 |
| Title |
Yeast_12h in 2% glucose_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast cells which were cultured for 12h in 2% glucose-containing YPD media
|
| Organism |
Saccharomyces cerevisiae BY4741 |
| Characteristics |
time point: 12h
protocol: 2% glucose-containing YPD media
|
| Treatment protocol |
Yeast harvested at indicated time-points was broken in RNA isolation buffer using a Beadbeater (BioSpec).
|
| Growth protocol |
Budding yeast cells were inoculated into liquid YPD media with an initial OD600 = 0.2 containing 2% and 0.5% glucose respectively. All cultures were incubated at 30 °C in a 200 rpm shaking incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For total RNA isolation, we used TRIzol LS reagent (Invitrogen), according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were synthesized according to the Affymetrix protocol from 250 ng of total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 μg of fragmented cRNA were hybridized onto a microarray (GeneChip Yeast Genome 2.0 Array) for 16h at 45 °C. Microarrays were washed and stained in GeneChip Fluidics Station 450.
|
| Scan protocol |
Microarrays were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from yeast cells which were cultured in 2% glucose-containing YPD media and harvested at 12h after inoculation.
|
| Data processing |
All raw data were generated using Affymetrix software, Command Console. The cel files were imprted into Partek Discovery Suite and further processed using quantile normalization in Partek Discovery Suite.
|
| |
|
| Submission date |
Oct 26, 2012 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Cheol-Koo Lee |
| E-mail(s) |
cklee2005@korea.ac.kr
|
| Phone |
82-2-3290-3008
|
| Organization name |
Korea University
|
| Department |
Biotechnology
|
| Lab |
Functional Genomics
|
| Street address |
Korea University, Biotechnology #318 West building, Anam-dong, Seongbuk-gu
|
| City |
Seoul |
| ZIP/Postal code |
136-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE41860 |
Time-course expression data during assurance of the chronological longevity by caloric restriction in budding yeast |
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