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Status |
Public on Dec 31, 2013 |
Title |
Yeast_18h in 2% glucose_rep1 |
Sample type |
RNA |
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Source name |
Yeast cells which were cultured for 18h in 2% glucose-containing YPD media
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Organism |
Saccharomyces cerevisiae BY4741 |
Characteristics |
time point: 18h protocol: 2% glucose-containing YPD media
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Treatment protocol |
Yeast harvested at indicated time-points was broken in RNA isolation buffer using a Beadbeater (BioSpec).
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Growth protocol |
Budding yeast cells were inoculated into liquid YPD media with an initial OD600 = 0.2 containing 2% and 0.5% glucose respectively. All cultures were incubated at 30 °C in a 200 rpm shaking incubator.
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Extracted molecule |
total RNA |
Extraction protocol |
For total RNA isolation, we used TRIzol LS reagent (Invitrogen), according to the manufacturer's instructions.
|
Label |
Biotin
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Label protocol |
Biotinylated cRNA were synthesized according to the Affymetrix protocol from 250 ng of total RNA.
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Hybridization protocol |
Following fragmentation, 5 μg of fragmented cRNA were hybridized onto a microarray (GeneChip Yeast Genome 2.0 Array) for 16h at 45 °C. Microarrays were washed and stained in GeneChip Fluidics Station 450.
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Scan protocol |
Microarrays were scanned using the GeneChip Scanner 3000.
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Description |
Gene expression data from yeast cells which were cultured in 2% glucose-containing YPD media and harvested at 18h after inoculation.
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Data processing |
All raw data were generated using Affymetrix software, Command Console. The cel files were imprted into Partek Discovery Suite and further processed using quantile normalization in Partek Discovery Suite.
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Submission date |
Oct 26, 2012 |
Last update date |
Dec 31, 2013 |
Contact name |
Cheol-Koo Lee |
E-mail(s) |
cklee2005@korea.ac.kr
|
Phone |
82-2-3290-3008
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Organization name |
Korea University
|
Department |
Biotechnology
|
Lab |
Functional Genomics
|
Street address |
Korea University, Biotechnology #318 West building, Anam-dong, Seongbuk-gu
|
City |
Seoul |
ZIP/Postal code |
136-701 |
Country |
South Korea |
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|
Platform ID |
GPL2529 |
Series (1) |
GSE41860 |
Time-course expression data during assurance of the chronological longevity by caloric restriction in budding yeast |
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