|
Status |
Public on Nov 09, 2006 |
Title |
In vitro erythroid differentiation at day 9, biological replicate 3 |
Sample type |
RNA |
|
|
Source name |
Human adult-derived peripheral blood CD34+ hematopoietic progenitor cells
|
Organism |
Homo sapiens |
Characteristics |
Human adult
|
Treatment protocol |
CD34+ cells were cultured in serum-free medium in the presence of dexamethasone (1µM), holo transferrin (0.3 mg/ml), erythropoietin (4 unit/ml), stem cell factor (10 ng/ml) and interleukin 3 (10 ng/ml).
|
Growth protocol |
Cells cultured for 11 days with samples at days 1, 3, 5, 7, 9 and 11.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by Qiagen RNAeasy micro kit according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cDNA was prepared from 50 ng total RNA using Ovation biotin RNA amplification and labeling system.
|
|
|
Hybridization protocol |
Fragmented and biotin-labeled 2.5 µg target in 200 µl hybridization cocktail was used for each Affymetrix U133 Plus 2.0 array.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Gene-Array scanner 3000
|
Description |
Gene expression data from human adult-derived peripheral blood CD34+ hematopoietic progenitor cells after in vitro erythroid differentiation for 11 days with samples at days 1, 3, 5, 7, 9 and 11
|
Data processing |
The data were analyzed with Gene Chip Operating Software (GCOS v3.0) using Affymetrix default analysis settings.
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|
|
Submission date |
Mar 31, 2006 |
Last update date |
Jul 17, 2007 |
Contact name |
Sankar Addya |
E-mail(s) |
sankar.addya@jefferson.edu
|
Organization name |
Thomas Jefferson University Hospital
|
Department |
Kimmel Cancer Center
|
Lab |
Cancer Genomics Lab
|
Street address |
233 South 10th Street
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19107 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE4655 |
Timecourse of in vitro erythroid differentiation of human adult-derived peripheral blood CD34+ cells |
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