|
| Status |
Public on Nov 09, 2006 |
| Title |
In vitro erythroid differentiation at day 9, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human adult-derived peripheral blood CD34+ hematopoietic progenitor cells
|
| Organism |
Homo sapiens |
| Characteristics |
Human adult
|
| Treatment protocol |
CD34+ cells were cultured in serum-free medium in the presence of dexamethasone (1µM), holo transferrin (0.3 mg/ml), erythropoietin (4 unit/ml), stem cell factor (10 ng/ml) and interleukin 3 (10 ng/ml).
|
| Growth protocol |
Cells cultured for 11 days with samples at days 1, 3, 5, 7, 9 and 11.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Qiagen RNAeasy micro kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 50 ng total RNA using Ovation biotin RNA amplification and labeling system.
|
| |
|
| Hybridization protocol |
Fragmented and biotin-labeled 2.5 µg target in 200 µl hybridization cocktail was used for each Affymetrix U133 Plus 2.0 array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene-Array scanner 3000
|
| Description |
Gene expression data from human adult-derived peripheral blood CD34+ hematopoietic progenitor cells after in vitro erythroid differentiation for 11 days with samples at days 1, 3, 5, 7, 9 and 11
|
| Data processing |
The data were analyzed with Gene Chip Operating Software (GCOS v3.0) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Apr 07, 2006 |
| Last update date |
Jul 17, 2007 |
| Contact name |
Sankar Addya |
| E-mail(s) |
sankar.addya@jefferson.edu
|
| Organization name |
Thomas Jefferson University Hospital
|
| Department |
Kimmel Cancer Center
|
| Lab |
Cancer Genomics Lab
|
| Street address |
233 South 10th Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE4655 |
Timecourse of in vitro erythroid differentiation of human adult-derived peripheral blood CD34+ cells |
|
