|
Status |
Public on Nov 21, 2014 |
Title |
LUMA-23 |
Sample type |
RNA |
|
|
Source name |
Breast tumor
|
Organism |
Homo sapiens |
Characteristics |
er: 1 pgr: 1 her2: 0 ki67: 10 grade: 1 tissue: breast cancer tumor
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated by the Trizol method (Invitrogen) according to manufacturers’ instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Rev. 5, Affymerrix)
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|
|
Hybridization protocol |
Hybridization was done according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Rev. 5, Affymetrix)
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner
|
Data processing |
The probe level intensities were background adjusted using the Robust multi-array analysis (RMA) method followed by quantile normalization as impletemented in the R/Bioconductor package frma. Probe level annotations were obtained from Affymetrix’s website. In particular, we used release 31 of the NetAffx annotation files.
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|
|
Submission date |
Jan 08, 2013 |
Last update date |
Nov 22, 2014 |
Contact name |
David Brown |
E-mail(s) |
david.brown@bordet.be
|
Phone |
+3225413743
|
Organization name |
Institut Jules Bordet
|
Lab |
J.-C. Heuson Breast Cancer Translational Research Laboratory
|
Street address |
Boulevard de Waterloo 127
|
City |
Brussels |
ZIP/Postal code |
1000 |
Country |
Belgium |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE43358 |
Transfer of clinically relevant gene expression signatures in breast cancer: from Affymetrix microarray to Illumina RNA-Sequencing technology |
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