|
| Status |
Public on Dec 31, 2013 |
| Title |
sage overexpression_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: embryonic stages 11-16
genotype/variation: sage overexpressed tub-Gal4
|
| Treatment protocol |
Embryos were sorted on a COPAS biosorter prior to RNA extraction.
|
| Growth protocol |
Embryos were collected and aged at 25 C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by TriZol extraction and cleaned up using the Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Amplification and labeling were performed using the Affymetrix GeneChip3' IVT express kit (P/N 901229) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples and controls were prepared using the Affymetrix GeneChip hybridization, wash and stain kit (P/N 900720). Arrays were prehybridized for 10 minutes prior to sample addition. Following sample addition, arrays were hybridized for 16 hours at 45 Celsius, and shaken at 60 rpm. Arrays were stained and washed using the Affymetrix GeneChip Fluidics Station 450 and FS450_002 fluidics script.
|
| Scan protocol |
Arrays were scanned with the Affymetrix GeneChip Scanner 3000 and raw analysis was performed with Affymetrix Command Console and Expression Console.
|
| Data processing |
Data was normalized by RMA and statistical analysis was performed using Partek software. Further analysis was performed using Spotfire software.
|
| |
|
| Submission date |
Feb 05, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Deborah J Andrew |
| E-mail(s) |
dandrew@jhmi.edu
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Cell Biology
|
| Street address |
725 N. Wolfe St., Hunterian G-1
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE44090 |
Genes regulated by ectopic expression of Sage in the entire Drosophila embryo using RNA from tub-Gal4 embryos. |
|
