|
Status |
Public on Feb 07, 2013 |
Title |
Control M1_SH-SY5Y [miRNA] |
Sample type |
RNA |
|
|
Source name |
Human Neuronal Cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: SH-SY5Y neuronal cell line treatment: Control
|
Treatment protocol |
Cells were treated with 10 pg/ml of Vpr protein kindly received from Dr Eric Cohen, University of Montreal-Canada or with 10 ng/ml of Tat protein kindly received from NIH (AIDS reagnets Bank) for 24 hours.
|
Growth protocol |
Neuronal cell line, SH-SY5Y (5 x 105) were grown in DMEM + 10% FBS on MatTek glass bottom plates treated with collagen (MatTek, Ashland, MA).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen, Carlsbad, CA). 750 ng of total RNA was used for miRNA array analysis using miRCURY LNATM microRNA Array, v. 11.0 (Exiqon, Woburn, MA).
|
Label |
Hy5
|
Label protocol |
Standard Agilent protocol.
|
|
|
Hybridization protocol |
Standard Agilent protocol.
|
Scan protocol |
Array chips were scanned using Axon GenePix Scanner (Molecular Devices, Downingtown, PA) and Genepix 4000 image capture software (Molecular Devices, Downingtown, PA).
|
Data processing |
Quantile normalization was used using JMP Genomics (JMP, Cary, NC) at Harvard Catalyst - Laboratory for Innovative Translational Technology (HC-LITT) and differentially regulated miRNAs were determined. Untreated cells were used as control.
|
|
|
Submission date |
Feb 07, 2013 |
Last update date |
Feb 12, 2013 |
Contact name |
Bassel E Sawaya |
E-mail(s) |
sawaya@temple.edu
|
Phone |
2157075446
|
Organization name |
Temple University
|
Department |
Neurology
|
Street address |
3307 N. Broad Street
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19140 |
Country |
USA |
|
|
Platform ID |
GPL8227 |
Series (1) |
GSE44265 |
HIV-1 Tat protein promotes neuronal dysfunction through disruption of microRNAs. |
|