|
| Status |
Public on Nov 12, 2013 |
| Title |
Fibroblast-NCsiRNA-72hr-lot2 |
| Sample type |
RNA |
| |
|
| Source name |
Fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Fibroblast
treatment: 72hr after NCsiRNA transfection
|
| Treatment protocol |
siRNA transfection; final 50nM of siRNA was transfected to fibroblast with lipofectamin RNAiMAX and OptiMEM according to manufacturer instruction. Medium was changed 48hr after transfection. Then adipogenic induction was started 24hr after medium change.
|
| Growth protocol |
Culture condition and adipogenic condition of MSC and preadipocyte were archieved according to Lonza recommended protocol. Culturecondition of human deraml fibroblast was minimum essential medium (MEM-α) supplemented with 10% fetal bovine serum (FBS), 10ml of L-Glutamine and 0.5 ul of antibiotics (Takara Inc).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with TotalPrep™ RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridyzation protocol
|
| Scan protocol |
BeadArray Reader
|
| Description |
Replicate2
5713317097_K
|
| Data processing |
Genomestudio V2011.1
|
| |
|
| Submission date |
Feb 13, 2013 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Yasuhiro Tomaru |
| E-mail(s) |
tomaru@gsc.riken.jp
|
| Organization name |
RIKEN
|
| Department |
Omics Science Center
|
| Street address |
1-7-22 Suehicho
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE44303 |
KD of Fibroblast specific TF |
| GSE44439 |
Basic data set for advanced transdifferentiation from fibroblast |
|
