|
Status |
Public on Jan 29, 2014 |
Title |
forebrain_hom_female_rep2 |
Sample type |
RNA |
|
|
Source name |
forebrain, female, homozygote
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6J genotype: homozygote age: E12.5 Sex: female tissue: forebrain mouse id: 542_E5
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kits in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol using HiScan.
|
Description |
Replicate 2
|
Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio 2011.1.
|
|
|
Submission date |
Feb 22, 2013 |
Last update date |
Jan 29, 2014 |
Contact name |
Martin Irmler |
Organization name |
Helmholtz Zentrum München GmbH
|
Department |
Institute of Experimental Genetics
|
Lab |
Gene Regulation & Epigenetics
|
Street address |
Ingolstaedter Landstrasse 1
|
City |
Neuherberg |
State/province |
Bayern |
ZIP/Postal code |
85764 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE44592 |
Restless Legs Syndrome-associated variant in MEIS1 confers altered gene expression in embryonic ganglionic eminences |
|