cell type: breast cancer cell line cell line: MB231RN-LM, derived from MDA-MB-231 genotype/variation: transfected with GFP (control)
Treatment protocol
cells were untreated or treated with CoCL2 (200uM, 4h)
Growth protocol
cells were maintained in gelatin-coated flasks in DMEM/F12 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 30% human foreskin fibroblast conditioned medium, 100 units/ml penicillin, 100 µg/ml streptomycin, 10 mg/ml fungin (InvivoGen, San Diego, CA) and 400 ug/ml G418.
Extracted molecule
total RNA
Extraction protocol
Total RNA was prepared using RNeasy mini kit (Qiagen) according to the manufacturer's instructions
Label
Biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Illumina protocol
Hybridization protocol
Biotinylated cRNA were hybridized to the Illumina array per manufacturer's instructions
Scan protocol
Standard Illumina scanning protocol
Description
replicate 1 7018861076_C
Data processing
Illumina Genome Studio software was used for data processing: background subtraction and Quantile normalization
