|
| Status |
Public on Aug 26, 2015 |
| Title |
MALME-3M_replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
melanoma cell line MALME-3M
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: melanoma cell line
cell line: melanoma cell line MALME-3M
|
| Growth protocol |
Cell lines were plated and harvested when 50-80% confluent. RNA was extracted and hybridized on Affymetrix microarrays. At least 2 biological repeats were performed for each cell line.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1mg total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was for 16hours andaccording to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450 using standard Fluidics Protocol (Fluidics protocol FS450_007). GeneChips were scanned using the Affymetrix 7G Scanner.
|
| Description |
gene expression data from melanoma cell line MALME-3M
|
| Data processing |
The data was rma normalised using the R Bioconductor package affy.
|
| |
|
| Submission date |
Mar 27, 2013 |
| Last update date |
Aug 26, 2015 |
| Contact name |
Petranel T Ferrao |
| E-mail(s) |
pferrao@unimelb.edu.au
|
| Phone |
61 3 9656 1806
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Cancer Research
|
| Street address |
St. Andrew's Place
|
| City |
East Melbourne |
| ZIP/Postal code |
3125 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE45558 |
Expression data from BRAF-mutant melanoma cell lines |
|
