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Status |
Public on Mar 15, 2015 |
Title |
MCF7_vector_vehicle_6h, rep2 |
Sample type |
RNA |
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|
Source name |
MCF7 cells stably expressing plasmid vector, treated with ethanol vehicle, for 6 hours
|
Organism |
Homo sapiens |
Characteristics |
parental cell line: MCF7 tissue derivation: breast cancer stable vector integration: pSG5-empty treatment: ethanol vehicle time point: 6 hr
|
Treatment protocol |
Samples were treated in IMEM medium, with or without 10e-7 M estradiol for 6 hr.
|
Growth protocol |
Samples were grown in complete medium (MEM, 5% FBS, 1% NEAA, 1% pen/strep, 6ng/ml insulin, 200 ug/ml G418) before 1 day starvation in IMEM medium, then treated in IMEM medium for 6 hr.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
pSG5.EtOH.2 Replicate 2 of 3.
|
Data processing |
The data were filtered and any probes with a detection P-value higher than 0.05 were removed. The data were then normalized using log2 transformation and quantile normalization with R using the lumi Bioconductor package.
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|
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Submission date |
Mar 29, 2013 |
Last update date |
Mar 15, 2015 |
Contact name |
Todd P Knutson |
E-mail(s) |
knut0297@umn.edu
|
Phone |
612-626-8911
|
Organization name |
University of Minnesota
|
Department |
Minnesota Supercomputing Institute
|
Street address |
117 Pleasant St SE
|
City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE45643 |
Progesterone receptor-B enhances estrogen responsiveness of breast cancer cells via scaffolding PELP1- and estrogen receptor-containing transcription complexes |
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