|
| Status |
Public on Apr 18, 2013 |
| Title |
K422 aza/tsa |
| Sample type |
RNA |
| |
|
| Source name |
cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: K422
tissue: B-cell lymphoma cell lines
treatment: aza/tsa
|
| Growth protocol |
72h growth with every 24h adding aza, last 12 h adding TSA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
DIG
|
| Label protocol |
Standard one-round amplification was performed using the NanoAmp RT-IVT Labeling Kit (Applied Biosystems) according to the manufacturers' protocol. Briefly, cDNA was synthesized from one g total RNA in an oligo dT primed reaction. Labeled complementary RNA (cRNA) was obtained from double-stranded cDNA in the presence of digoxigenin (DIG)-UTP in an in vitro labeling reaction.
|
| |
|
| Hybridization protocol |
Applied Biosystems manufactures protocol
|
| Scan protocol |
Chemoluminescense was measured using the AB1700 Chemoluminescense Analyzer (Applied Biosystems) after incubating the array with alkalic phosphatase-linked digoxygenin antibody.
|
| Data processing |
Values represent the normalized expression after running ABarray on R-script.
|
| |
|
| Submission date |
Apr 15, 2013 |
| Last update date |
Apr 18, 2013 |
| Contact name |
Nicole Bethge |
| E-mail(s) |
nicbet@rr-research.no
|
| Organization name |
Institute for Cancer Research
|
| Department |
Immunology
|
| Street address |
Montebello veien
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE46064 |
Epigenetic treatment of B-cell lymphoma cell lines |
|
