|
Status |
Public on Apr 18, 2013 |
Title |
Sudhl6 aza/tsa |
Sample type |
RNA |
|
|
Source name |
cell lines
|
Organism |
Homo sapiens |
Characteristics |
cell line: Sudhl6 tissue: B-cell lymphoma cell lines treatment: aza/tsa
|
Growth protocol |
72h growth with every 24h adding aza, last 12 h adding TSA
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
DIG
|
Label protocol |
Standard one-round amplification was performed using the NanoAmp RT-IVT Labeling Kit (Applied Biosystems) according to the manufacturers' protocol. Briefly, cDNA was synthesized from one g total RNA in an oligo dT primed reaction. Labeled complementary RNA (cRNA) was obtained from double-stranded cDNA in the presence of digoxigenin (DIG)-UTP in an in vitro labeling reaction.
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|
|
Hybridization protocol |
Applied Biosystems manufactures protocol
|
Scan protocol |
Chemoluminescense was measured using the AB1700 Chemoluminescense Analyzer (Applied Biosystems) after incubating the array with alkalic phosphatase-linked digoxygenin antibody.
|
Data processing |
Values represent the normalized expression after running ABarray on R-script.
|
|
|
Submission date |
Apr 15, 2013 |
Last update date |
Apr 18, 2013 |
Contact name |
Nicole Bethge |
E-mail(s) |
nicbet@rr-research.no
|
Organization name |
Institute for Cancer Research
|
Department |
Immunology
|
Street address |
Montebello veien
|
City |
Oslo |
ZIP/Postal code |
0310 |
Country |
Norway |
|
|
Platform ID |
GPL2986 |
Series (1) |
GSE46064 |
Epigenetic treatment of B-cell lymphoma cell lines |
|