|
Status |
Public on Dec 31, 2013 |
Title |
monocyte from donor 4 stimulated with ATRA |
Sample type |
RNA |
|
|
Source name |
primary human monocytes isolated via plastic aherence from peripheral blood mononuclear cells.
|
Organism |
Homo sapiens |
Characteristics |
disease state: healthy cell type: monocyte individual: donor 4 treatment: all-trans retinoic acid
|
Treatment protocol |
enriched monocytes were treated with ATRA or 1,25D3 for 18hours at 10-8M
|
Growth protocol |
primary human monocytes enriched via plastic adherence from peripherial blood mononuclear cells derived from whole blood of healthy donors
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted using Trizol reagent based on the manufacturer's recommended protocol, and purified using RNAeasy RNA cleanup protocol according to the manufacturer's recommended protocol
|
Label |
biotin
|
Label protocol |
Standard labeling protocol by UCLA Clinical microarray core facility
|
|
|
Hybridization protocol |
Standard Affymetrix protocol by UCLA Clinical microarray core facility
|
Scan protocol |
Standard Affymetrix protocol by UCLA Clinical microarray core facility
|
Data processing |
UCLA DNA microarray core facility, normalized using MAS5
|
|
|
Submission date |
Apr 22, 2013 |
Last update date |
Dec 31, 2013 |
Contact name |
Philip T. Liu |
E-mail(s) |
ptliu@mednet.ucla.edu
|
Phone |
310-206-8457
|
Organization name |
University of California at Los Angeles
|
Department |
Department of Orthopaedic Surgery
|
Lab |
Liu Lab
|
Street address |
615 Charles E Young Drive South, 410 OHRC
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE46268 |
Gene expression profile of human monocytes stimulated with all-trans retinoic acid (ATRA) or 1,25a-dihydroxyvitamin D3 (1,25D3) |
|