|
| Status |
Public on Dec 31, 2013 |
| Title |
monocyte from donor 1 stimulated with 1,25D |
| Sample type |
RNA |
| |
|
| Source name |
primary human monocytes isolated via plastic aherence from peripheral blood mononuclear cells.
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: healthy
cell type: monocyte
individual: donor 1
treatment: 1,25a-dihydroxyvitamin D3
|
| Treatment protocol |
enriched monocytes were treated with ATRA or 1,25D3 for 18hours at 10-8M
|
| Growth protocol |
primary human monocytes enriched via plastic adherence from peripherial blood mononuclear cells derived from whole blood of healthy donors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Trizol reagent based on the manufacturer's recommended protocol, and purified using RNAeasy RNA cleanup protocol according to the manufacturer's recommended protocol
|
| Label |
biotin
|
| Label protocol |
Standard labeling protocol by UCLA Clinical microarray core facility
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol by UCLA Clinical microarray core facility
|
| Scan protocol |
Standard Affymetrix protocol by UCLA Clinical microarray core facility
|
| Data processing |
UCLA DNA microarray core facility, normalized using MAS5
|
| |
|
| Submission date |
Apr 22, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Philip T. Liu |
| E-mail(s) |
ptliu@mednet.ucla.edu
|
| Phone |
310-206-8457
|
| Organization name |
University of California at Los Angeles
|
| Department |
Department of Orthopaedic Surgery
|
| Lab |
Liu Lab
|
| Street address |
615 Charles E Young Drive South, 410 OHRC
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE46268 |
Gene expression profile of human monocytes stimulated with all-trans retinoic acid (ATRA) or 1,25a-dihydroxyvitamin D3 (1,25D3) |
|
