|
| Status |
Public on Jan 03, 2014 |
| Title |
TT2_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
TT2 Wildtype
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mESC
strain: C57BL/ 6 female and a CBA male
|
| Growth protocol |
mESCs cells were maintained in DMEM containing 15% FBS, penicillin/streptomycin and sodium pyruvate (PAA), 0.1 mM β-mercaptoethanol (Gibco), Non-Essential Amino acids (PAA) and 1000 U/mL of LIF ESGRO (PAA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Data processing |
The data were RMA normalized and analysed with Partek Genomic Suite. The custom CDF file is based on Ensembl genes (from http://brainarray.mbni.med.umich.edu version 15).
|
| |
|
| Submission date |
May 01, 2013 |
| Last update date |
Jan 03, 2014 |
| Contact name |
PONTIS Julien |
| E-mail(s) |
julien.pontis@univ-paris-diderot.fr, julien.pontis35@gmail.com
|
| Organization name |
UMR7216 CNRS
|
| Street address |
35, rue Hélène BRION
|
| City |
Paris |
| ZIP/Postal code |
75205 |
| Country |
France |
| |
|
| Platform ID |
GPL17103 |
| Series (2) |
| GSE46544 |
The Histone H3 Lysine 9 Methyltransferases G9a and GLP Regulate Polycomb Repressive Complex 2-Mediated Gene Silencing [Affymetrix] |
| GSE46545 |
The Histone H3 Lysine 9 Methyltransferases G9a and GLP Regulate Polycomb Repressive Complex 2-Mediated Gene Silencing |
|
