|
Status |
Public on Feb 27, 2014 |
Title |
T47D_PRB_unsynch_vehicle_6h_rep3 |
Sample type |
RNA |
|
|
Source name |
T47D cells stably expressing PR-B vector, unsynchronized, treated with ethanol vehicle for 6 hours
|
Organism |
Homo sapiens |
Characteristics |
parental cell line: T47D-YB tissue derivation: breast cancer stable vector integration: pSG5-PR-B cell synchronization: vehicle treatment: ethanol vehicle time point: 6 hr
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
PRB.unsynch.EtOH.3 Replicate 3 of 3.
|
Data processing |
The data were quantile normalized using Genedata Analyst software using default settings, but are not log2 transformed.
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|
|
Submission date |
May 07, 2013 |
Last update date |
Feb 27, 2014 |
Contact name |
Todd P Knutson |
E-mail(s) |
knut0297@umn.edu
|
Phone |
612-626-8911
|
Organization name |
University of Minnesota
|
Department |
Minnesota Supercomputing Institute
|
Street address |
117 Pleasant St SE
|
City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
|
|
Platform ID |
GPL10904 |
Series (1) |
GSE46715 |
Progesterone Receptor–Cyclin D1 Complexes Induce Cell Cycle–Dependent Transcriptional Programs in Breast Cancer Cells |
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