|
| Status |
Public on Feb 27, 2014 |
| Title |
T47D_PRB_synch_R5020_6h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
T47D cells stably expressing PR-B vector, synchronized at G2/M with nocodazole, treated with R5020 vehicle for 6 hours
|
| Organism |
Homo sapiens |
| Characteristics |
parental cell line: T47D-YB
tissue derivation: breast cancer
stable vector integration: pSG5-PR-B
cell synchronization: nocodazole
treatment: R5020 10e-8 M
time point: 6 hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
PRB.synch.R5020.2
Replicate 2 of 3.
|
| Data processing |
The data were quantile normalized using Genedata Analyst software using default settings, but are not log2 transformed.
|
| |
|
| Submission date |
May 07, 2013 |
| Last update date |
Feb 27, 2014 |
| Contact name |
Todd P Knutson |
| E-mail(s) |
knut0297@umn.edu
|
| Phone |
612-626-8911
|
| Organization name |
University of Minnesota
|
| Department |
Minnesota Supercomputing Institute
|
| Street address |
117 Pleasant St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE46715 |
Progesterone Receptor–Cyclin D1 Complexes Induce Cell Cycle–Dependent Transcriptional Programs in Breast Cancer Cells |
|
