|
Status |
Public on Dec 25, 2013 |
Title |
PrEC LH cells NKX3.1 7h rep2 |
Sample type |
RNA |
|
|
Source name |
Human prostate epithelium
|
Organism |
Homo sapiens |
Characteristics |
tissue: prostate epithelium expression construct: Ad-GFP-NKX3.1 expression time hours: 7
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2 1705168246_B
|
Data processing |
The data were filtered for p> 0.01 and normalised using quantile normalisation with GeneSpring
|
|
|
Submission date |
May 16, 2013 |
Last update date |
Dec 25, 2013 |
Contact name |
Roy M Williams |
E-mail(s) |
rwillia@scripps.edu
|
Organization name |
The Scripps Research Institute
|
Department |
Regenerative Medicine
|
Lab |
Loring
|
Street address |
3030 Science Park Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL6171 |
Series (1) |
GSE47030 |
Systems analysis of the prostate tumor suppressor NKX3.1 substantiates roles in DNA repair and a transcriptional program that drives luminal cell differentiation |
|