|
| Status |
Public on Dec 25, 2013 |
| Title |
PrEC LH cells NKX3.1 10h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human prostate epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate epithelium
expression construct: Ad-GFP-NKX3.1
expression time hours: 10
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
1705168246_C
|
| Data processing |
The data were filtered for p> 0.01 and normalised using quantile normalisation with GeneSpring
|
| |
|
| Submission date |
May 16, 2013 |
| Last update date |
Dec 25, 2013 |
| Contact name |
Roy M Williams |
| E-mail(s) |
rwillia@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
3030 Science Park Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6171 |
| Series (1) |
| GSE47030 |
Systems analysis of the prostate tumor suppressor NKX3.1 substantiates roles in DNA repair and a transcriptional program that drives luminal cell differentiation |
|
