|
| Status |
Public on Dec 23, 2013 |
| Title |
TIBC1 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from frozen human breast tumor samples
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer
condition: true interval tumors (TIBC)
phenotype: TN
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following Qiagen protocol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100-300 ng of total RNA were amplified and labeled using the WT Sense Target labeling system and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix Human Gene 1.0 ST array using a Affymetrix GeneChip Hybridization Oven 645
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from from human breast tumor
|
| Data processing |
The data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between samples
|
| |
|
| Submission date |
May 20, 2013 |
| Last update date |
Dec 23, 2013 |
| Contact name |
Ana Rovira |
| E-mail(s) |
arovira@imim.es
|
| Organization name |
IMIM-Hospital del Mar
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE47108 |
Gene expression profiling in true interval breast cancer reveals overactivation of mTOR signalling pathway |
|
