|
| Status |
Public on May 22, 2013 |
| Title |
hmNPCs_HD-HAd_2h_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
hmNPCs cells, transduction, HD-HAd, 2h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human midbrain neuroprogenitor cells
tissue: embryonic midbrain tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by using the RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
labeled cRNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays according to the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
Arrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix) according to the manufacturer's protocol.
|
| Description |
Gene expression data from hmNPCs cells transduced with HD-HAd and collection at 2 hour
|
| Data processing |
Expression data (CEL files) were analysed by the Robust Multiarray Average (RMA) method of Irrizarry using the R/Bioconductor suite of packages.
|
| |
|
| Submission date |
May 21, 2013 |
| Last update date |
May 22, 2013 |
| Contact name |
Valerio Licursi |
| E-mail(s) |
valerio.licursi@uniroma1.it
|
| Organization name |
Institute of Molecular Biology and Pathology (IBPM), National Research Council (CNR)
|
| Department |
c/o Dept. Biology and Biotechnologies "C. Darwin", Sapienza University of Rome
|
| Street address |
via degli Apuli, 4
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE47130 |
Vector-transduced neurons transcriptome profiles |
|
