|
| Status |
Public on May 02, 2014 |
| Title |
Pancreatic islets from Pdx-1+/- mice 2 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreatic islets isolated from Pdx-1+/- mice (Pdx-1-tTA is generating Pdx-1 haploinsufficiency)
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mouse pancreatic islets
strain: C57BL/6 and NMRI (1:1)
|
| Treatment protocol |
Mice were left untreated prior to islet isolation.
|
| Growth protocol |
Mice were housed under specific pathogen-free conditions at the animal facility of the University of Ulm and fed ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from pancreatic islet with RNeasy mini kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
The total RNAs were amplified and labeled following the Whole Transcript (WT) Sense Target Labeling Assay (http://www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Labeled ssDNA was hybridized to Mouse Gene 1.0 ST Affymetrix GeneChip arrays (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
The chips were scanned with a Affymetrix GeneChip Scanner 3000 according to the Affimetrix protocol.
|
| Description |
Gene expression data from pancreatic islets of Pdx-1+/- mice
|
| Data processing |
Affymetrix® Expression Console™ Software for normalization, BRB Array Tools (http://linus.nci.nih.gov/BRB-ArrayTools.html) and GeneSifter software (http://www.geospiza.com) for further analysis
|
| |
|
| Submission date |
May 30, 2013 |
| Last update date |
May 02, 2014 |
| Contact name |
Karlheinz Holzmann |
| E-mail(s) |
karlheinz.holzmann@uni-ulm.de
|
| Organization name |
Ulm University
|
| Department |
Medical Faculty
|
| Lab |
Genomics Core Facility
|
| Street address |
Helmholtzstreet 8/1
|
| City |
Ulm |
| ZIP/Postal code |
D-89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47504 |
Gene expresssion changes in pancreatic islets of 11 weeks old IKK2-CApdx-1 mice compared to control and Pdx-1+/- mice |
|
