|
| Status |
Public on May 01, 2014 |
| Title |
RT112 |
| Sample type |
RNA |
| |
|
| Source name |
urothelial cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: urothelial cancer cell line RT112
|
| Biomaterial provider |
All 30 cell lines were obtained from MD Anderson Bladder SPORE Tissue Bank.
|
| Growth protocol |
Cell lines were cultured in 10% FBS modified Eagle's MEM with standard supplements at 37 °C in 5% CO2 incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by mirVANA miRNA Isolation Kit. Quality control was performed by NanoDrop ND-1000 and Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Illumina RNA amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 26
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Jun 16, 2013 |
| Last update date |
May 01, 2014 |
| Contact name |
Tiewei Cheng |
| E-mail(s) |
tcheng@mdanderson.org
|
| Organization name |
UT MD Anderson Cancer Center
|
| Street address |
7777 Knight Rd.
|
| City |
77054 |
| State/province |
United States |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE47992 |
Genome wide expression profiling of 30 urothelial cancer cells |
| GSE48277 |
Identification of distinct basal and luminal subsets of human bladder cancers with different sensitivities to frontline chemotherapy |
|
