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Sample GSM1172820 Query DataSets for GSM1172820
Status Public on Jul 01, 2013
Title TC.VITRO.0hr#1
Sample type RNA
 
Source name Spleen
Organism Mus musculus
Characteristics age: 9 Weeks Old
inclusion markers: stained with B220-CD8a-CD11b-CD11c,-CD4+GFP-
strain: NOD
genotype/variation: Foxp3-IREs-GFP+/+
source prep: Splenocytes from a Foxp3-IRES-GFP mouse in culture
cell treatment: In vitro 1.25ug/ml of KT3 stimulation for 0hr
separation method: MoFlo Sorter
Sex: Female
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Affymetrix WT Sense Target Labeling Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Jun 21, 2013
Last update date Jul 01, 2013
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (1)
GSE48210 Kinetic analysis of the response to anti-CD3 in conventional and regulatory CD4+ T cells, in mice and in vitro.

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 37.0851
10344622 173.0056
10344624 90.8758
10344633 818.9477
10344637 82.2157
10344653 26.2787
10344658 39.3486
10344674 14.2615
10344679 59.08
10344707 78.8747
10344713 563.0307
10344723 243.3979
10344725 96.9356
10344741 1937.2508
10344743 57.3879
10344750 101.4611
10344772 44.8534
10344789 46.8334
10344797 374.2569
10344799 277.3721

Total number of rows: 24922

Table truncated, full table size 425 Kbytes.




Supplementary file Size Download File type/resource
GSM1172820_EA07068_108782_MoGene_TC1.250HR_1.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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