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Sample GSM1172829 Query DataSets for GSM1172829
Status Public on Jul 01, 2013
Title TR.VITRO.72hr#1
Sample type RNA
 
Source name Spleen
Organism Mus musculus
Characteristics age: 9 Weeks Old
inclusion markers: stained with B220-CD8a-CD11b-CD11c,-CD4+GFP-
strain: NOD
genotype/variation: Foxp3-IREs-GFP+/+
source prep: Splenocytes from a Foxp3-IRES-GFP mouse in culture
cell treatment: In vitro 1.25ug/ml of KT3 stimulation for 72hr
separation method: MoFlo Sorter
Sex: Female
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Affymetrix WT Sense Target Labeling Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Jun 21, 2013
Last update date Jul 01, 2013
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (1)
GSE48210 Kinetic analysis of the response to anti-CD3 in conventional and regulatory CD4+ T cells, in mice and in vitro.

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 19.4586
10344622 209.4366
10344624 834.0566
10344633 843.6969
10344637 250.8151
10344653 17.0574
10344658 150.7526
10344674 12.7186
10344679 49.4323
10344707 241.496
10344713 2839.823
10344723 222.4775
10344725 49.4728
10344741 3369.4958
10344743 27.7119
10344750 218.8253
10344772 27.6809
10344789 137.9598
10344797 279.2261
10344799 211.1263

Total number of rows: 24922

Table truncated, full table size 424 Kbytes.




Supplementary file Size Download File type/resource
GSM1172829_EA07068_108791_MoGene_TR1.2572HR_1.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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