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Sample GSM1172835 Query DataSets for GSM1172835
Status Public on Jul 01, 2013
Title TR.VITRO.0hr#2
Sample type RNA
 
Source name Spleen
Organism Mus musculus
Characteristics age: 14 Weeks Old
inclusion markers: stained with B220-CD8a-CD11b-CD11c,-CD4+GFP-
strain: NOD
genotype/variation: Foxp3-IREs-GFP+/+
source prep: Splenocytes from a Foxp3-IRES-GFP mouse in culture
cell treatment: In vitro 1.25ug/ml of KT3 stimulation for 0hr
separation method: MoFlo Sorter
Sex: Male
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Affymetrix WT Sense Target Labeling Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Jun 21, 2013
Last update date Jul 01, 2013
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (1)
GSE48210 Kinetic analysis of the response to anti-CD3 in conventional and regulatory CD4+ T cells, in mice and in vitro.

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 20.8812
10344622 202.587
10344624 626.7901
10344633 804.1466
10344637 257.4345
10344653 18.292
10344658 351.3484
10344674 14.6188
10344679 47.8026
10344707 455.0375
10344713 704.738
10344723 213.2153
10344725 45.6802
10344741 2804.5326
10344743 25.8859
10344750 311.0941
10344772 26.9019
10344789 203.6373
10344797 349.9296
10344799 246.9454

Total number of rows: 24922

Table truncated, full table size 424 Kbytes.




Supplementary file Size Download File type/resource
GSM1172835_EA07068_110611_MoGene_TR1.250HR_2.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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