|
| Status |
Public on Jul 01, 2013 |
| Title |
TR.VITRO.2hr#2 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
age: 14 Weeks Old
inclusion markers: stained with B220-CD8a-CD11b-CD11c,-CD4+GFP-
strain: NOD
genotype/variation: Foxp3-IREs-GFP+/+
source prep: Splenocytes from a Foxp3-IRES-GFP mouse in culture
cell treatment: In vitro 1.25ug/ml of KT3 stimulation for 2hr
separation method: MoFlo Sorter
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Affymetrix WT Sense Target Labeling Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Data processing |
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Jun 21, 2013 |
| Last update date |
Jul 01, 2013 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE48210 |
Kinetic analysis of the response to anti-CD3 in conventional and regulatory CD4+ T cells, in mice and in vitro. |
|
