|
| Status |
Public on Apr 21, 2014 |
| Title |
superior frontal gyrus_female_86_AD_15 |
| Sample type |
RNA |
| |
|
| Source name |
superior frontal gyrus_female_AD
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age (yrs): 86
brain region: superior frontal gyrus
braak stage: VI
apoe genotype: 4,4
mmse: 9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA, followed by purification using Qiagen quick-spin columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix) using robotics system (Biomek FX MicroArray Plex SA System; Beckman Coulter) to optimize consistency in processing
|
| |
|
| Hybridization protocol |
Following fragmentation, 30 mg biotin-labeled cRNA were hybridized for 16 hr and rotated at 13 rpm at 50° C on Affymetrix Human HgU133 plus 2.0 microarrays
|
| Scan protocol |
Affymetrix Genekeep GCS3000, software version 3.2.2.1515
|
| Description |
post-mortem tissue
|
| Data processing |
GC-RMA, using Genespring 7.3. data transformation: measurements <0.01 set to 0.01; per chip: normalized to the 50th percentile; per gene: normalize to median
|
| |
|
| Submission date |
Jun 27, 2013 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Nicole Claudia Berchtold |
| E-mail(s) |
nberchto@uci.edu
|
| Phone |
949 824 6071
|
| Organization name |
UC Irvine
|
| Department |
Institute for Memory Impairments and Neurological Disorders
|
| Lab |
Carl W. Cotman
|
| Street address |
1226 Gillespie Neuroscience Facility
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697-4540 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE133349 |
