|
| Status |
Public on Dec 01, 2013 |
| Title |
MDA468 37oC rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MDA468 37oC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA468
cell type: breast cancer cells
treatment: 37oC control
replicate: #1
|
| Growth protocol |
MCF10A, MCF7, MDA231, and MDA468 cells were maintained at constant 37oC as a control. For the experimental treatment, prewarmed (45oC) conditioned media was added to each cell line and the cells were incubated for 30 minutes. The 45oC media was then replaced with 37oC conditioned media. Cells were allowed to grow for 4 hours and RNA was subsequently extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was collected from each cell line 4 hours after completion of the hyperthermia treatment using the Qiagen RNAeasy mini kit.
|
| Label |
Cy3
|
| Label protocol |
RNA was amplified and biotin-labeled using Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
750 ng of biotinylated aRNA was briefly heat-denatured and loaded onto expression arrays to hybridize overnight. Following hybridization, arrays were labeled with Cy3-streptavidin.
|
| Scan protocol |
The chips were imaged on the Illumina ISCAN. Intensity values were transferred to GeneSpring GX microarray analysis software (Agilent).
|
| Data processing |
GeneSpring GX microarray analysis software (Agilent) was used to filter data based on quality of each call. Statistical relevance was determined using ANOVA with a Benjamini Hochberg FDR multiple testing correction (p-value < 0.05).
|
| |
|
| Submission date |
Jun 28, 2013 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Brad Allen Bryan |
| E-mail(s) |
brad.bryan@ttuhsc.edu
|
| Organization name |
Texas Tech University Health Sciences Center
|
| Department |
Biomedical Sciences
|
| Street address |
5001 El Paso Drive
|
| City |
El Paso |
| State/province |
Texas |
| ZIP/Postal code |
79905 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE48398 |
Gene expression profiles of mammary epithelial and breast cancer cells following fever range hyperthermia |
|
