|
| Status |
Public on Jul 13, 2013 |
| Title |
AdultTestes_aly[2]/aly[5p]_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
AdultTestes_aly[2]/aly[5p]
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: aly[2]/aly[5p] mutation
age: 0~1 day post eclosion
tissue: adult testes without seminal vesicles and accessory glands
|
| Treatment protocol |
Magn, WT(red, e), aly, and sa testes without seminal vesicle and accessory glands were dissected in icecold PBS containing 0.1% DEPC in 30 min intervals and immediately frozen in liquid nitrogen before transferred to -80°C. Total RNA was extracted using TRIzol (Invitrogen). Five micrograms of total RNA was processed for each replicate.
|
| Growth protocol |
Crosses and experimental flies were grown on cornmeal and molasses media at 25 °C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from ~250ng total RNA (GeneChip 3' IVT expression kit)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000-7G
|
| Description |
aly_2-5p_r1
|
| Data processing |
Data were background adjusted and normalized using R program (v2.13.2) with the BioConductor package GCRMA.
|
| |
|
| Submission date |
Jul 12, 2013 |
| Last update date |
Jul 13, 2013 |
| Contact name |
Jongmin J Kim |
| E-mail(s) |
jk2938@cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Biomedical Sciences
|
| Lab |
Kim lab
|
| Street address |
930 Campus Road
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE48837 |
Gene expression of fly testes with meiotic arrest from different mutations |
|
