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Sample GSM1185449 Query DataSets for GSM1185449
Status Public on Jul 13, 2013
Title AdultTestes_sa[1]/sa[2]_rep1
Sample type RNA
 
Source name AdultTestes_sa[1]/sa[2]
Organism Drosophila melanogaster
Characteristics genotype/variation: sa[1]/sa[2] mutation
age: 0~1 day post eclosion
tissue: adult testes without seminal vesicles and accessory glands
Treatment protocol Magn, WT(red, e), aly, and sa testes without seminal vesicle and accessory glands were dissected in icecold PBS containing 0.1% DEPC in 30 min intervals and immediately frozen in liquid nitrogen before transferred to -80°C. Total RNA was extracted using TRIzol (Invitrogen). Five micrograms of total RNA was processed for each replicate.
Growth protocol Crosses and experimental flies were grown on cornmeal and molasses media at 25 °C
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from ~250ng total RNA (GeneChip 3' IVT expression kit)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000-7G
Description sa_1-2_r1
Data processing Data were background adjusted and normalized using R program (v2.13.2) with the BioConductor package GCRMA.
 
Submission date Jul 12, 2013
Last update date Jul 13, 2013
Contact name Jongmin J Kim
E-mail(s) jk2938@cornell.edu
Organization name Cornell University
Department Biomedical Sciences
Lab Kim lab
Street address 930 Campus Road
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platform ID GPL1322
Series (1)
GSE48837 Gene expression of fly testes with meiotic arrest from different mutations

Data table header descriptions
ID_REF
VALUE log2 GC-RMA signal

Data table
ID_REF VALUE
1616608_a_at 11.2578521
1622892_s_at 10.24075748
1622893_at 10.32694413
1622894_at 2.232813073
1622895_at 8.321879476
1622896_at 7.667674266
1622897_at 2.116370016
1622898_a_at 11.09286129
1622899_at 2.213548595
1622900_at 2.101911293
1622901_at 2.124474809
1622902_at 2.14272546
1622903_s_at 6.751124431
1622904_at 2.533198634
1622905_at 2.101911293
1622906_at 4.817690893
1622907_at 6.488507891
1622908_a_at 4.552522173
1622909_at 6.949840906
1622910_at 2.101911293

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM1185449_sa_1-2_r1.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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