|
Status |
Public on Jul 13, 2013 |
Title |
AdultTestes_magn[12c]/magn[23b]_rep2 |
Sample type |
RNA |
|
|
Source name |
AdultTestes_magn[12c]/magn[23b]
|
Organism |
Drosophila melanogaster |
Characteristics |
genotype/variation: magn[12c]/magn[23b] mutation age: 0~1 day post eclosion tissue: adult testes without seminal vesicles and accessory glands
|
Treatment protocol |
Magn, WT(red, e), aly, and sa testes without seminal vesicle and accessory glands were dissected in icecold PBS containing 0.1% DEPC in 30 min intervals and immediately frozen in liquid nitrogen before transferred to -80°C. Total RNA was extracted using TRIzol (Invitrogen). Five micrograms of total RNA was processed for each replicate.
|
Growth protocol |
Crosses and experimental flies were grown on cornmeal and molasses media at 25 °C
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from ~250ng total RNA (GeneChip 3' IVT expression kit)
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000-7G
|
Description |
magn_12c-23b_r2
|
Data processing |
Data were background adjusted and normalized using R program (v2.13.2) with the BioConductor package GCRMA.
|
|
|
Submission date |
Jul 12, 2013 |
Last update date |
Jul 13, 2013 |
Contact name |
Jongmin J Kim |
E-mail(s) |
jk2938@cornell.edu
|
Organization name |
Cornell University
|
Department |
Biomedical Sciences
|
Lab |
Kim lab
|
Street address |
930 Campus Road
|
City |
Ithaca |
State/province |
NY |
ZIP/Postal code |
14853 |
Country |
USA |
|
|
Platform ID |
GPL1322 |
Series (1) |
GSE48837 |
Gene expression of fly testes with meiotic arrest from different mutations |
|